Evaluation of Moringa oleifera Seeds for Prophylactic and Curative Effects in CCl4 Induced Rat Liver Injury

The present research work was designed to establish the possible preventive and curative hepatoprotective efficacy on CCl4 poisoned rat liver toxicity. Fresh mature seeds were shade dried at room temperature, coarse powdered and extracted with 70% hydroalcohol by Soxhlet’s extraction method. Thereafter, the extract was concentrated using rotary flash evaporator to obtain semisolid crude extract with the yield of 10.52%. Moringa oleifera seeds extract was investigated in graded doses (100, 250 and 500 mg/kg) on CCl4 mediated liver injury in rats. Antihepatotxic potential was assessed by the estimation of biochemical markers viz. SGPT, SGOT, ALP, ACP and bilirubin (Direct & Total). In addition, liver GSH and MDA levels and histopathological examination were also studied. The dose dependent significant reduction of biochemical markers and bilirubin levels were seen in rats subjected to both pre and post treatments of test extract. Though, the extract at a dose of 100 mg/kg exhibited considerable reduction in concentration of ACP, TB and DB in pre and post treatment modes, but the results were found to be statistically not significant. Hepatic control rats showed significant decrease in hepatic GSH content and elevation of MDA (Malondialdehyde) level compared to normal control group. Pre and post treatment of text extract dose dependently reversed the CCl4 mediated altered hepatic GSH and MDA levels. The histopathological examination of rat liver sections were supported the hepatoprotective activity of the test extract. The findings of the present investigation suggest that seeds extract of the title plant possesses equally potent prophylactic and curative hepatoprotective efficacy against CCl4 rendered liver injury in rats.